Ginger and echinacea extracts improve the quality and fertility potential of frozen-thawed ram epididymal spermatozoa |
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| Institution: | 1. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran;2. Fertility & Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran;3. Infertility Treatment Research Center, Moatazedi Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran;1. Department of Basic Sciences, College of Education, Imam Abdulrahman Bin Faisal University, P.O. Box 2375, Dammam, 14513, Saudi Arabia;2. Basic and Applied Scientific Research Center, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia;3. Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia;4. Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia;5. Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt;6. Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia;7. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Thamar University, Yemen;8. Department of Animal Production, College of Agriculture and Veterinary Medicine, Thamar University, Yemen;1. Department of Animal Pathology, Reproduction and Obstetrics Area, Veterinary Faculty, Universidad de Zaragoza, Spain;2. Department of Animal Pathology, Infectious Diseases Area, Veterinary Faculty, Universidad de Zaragoza, Spain;1. Laboratorio de Biotecnología de la Reproducción Animal, Facultad de Ciencias Agropecuarias, Universidad de Cuenca, EC010205 Cuenca, Ecuador;2. Unidad Académica de Ciencias Agropecuarias, Carrera de Medicina Veterinaria, Universidad Católica de Cuenca, Cuenca, Ecuador;3. Departamento de Reproducción Animal, INIA-CSIC, 28040 Madrid, Spain |
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| Abstract: | The current study examined the impact of the supplementation of ginger and echinacea extract, as natural antioxidant agents, in freezing extender on the quality and fertility potential of ram epididymal spermatozoa after cryopreservation. Epididymal spermatozoa isolated from Forty testicles, obtained from 20 rams, with motility >80% and total morphological abnormalities <10% were pooled, divided into 7 aliquots and used for cryopreservation. The semen samples were re-suspended with basic Tris egg yolk diluent containing ginger and echinacea extracts (5, 10 and 20 mg/l). The control diluent comprised of only extender and lacked any antioxidant agent. For the determination of sperm quality, frozen straws were thawed after 7–10 days, and then the sperm characteristics were assessed. The supplementation of ginger at a concentration of 10 mg/l, as well as the addition of 10 and 20 mg/l echinacea extract significantly improved total motility and velocity parameters. The status of acrosome integrity and lipid peroxidation significantly improved in spermatozoa when supplemented with 10 mg/l ginger and 20 mg/l echinacea extract. Also, 5 mg/l ginger extract and 20 mg/l echinacea extract significantly improved mitochondrial activity. The highest ratio of the dispersion of sperm chromatin was observed in spermatozoa treated with 10 mg/l ginger extract. The cleavage rate was markedly higher in matured oocytes that were fertilized with frozen spermatozoa treated with 20 mg/l ginger extract and 10 mg/l echinacea. The application of ginger and echinacea extract resulted in improvement in the quality and fertility of frozen-thawed spermatozoa. However, future studies are wanted to elucidate how the active components in these extracts prevent cryo-damages in spermatozoa. |
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| Keywords: | Epididymal spermatozoa Echinacea extract Freezing extender Ginger extract In vitro fertilization |
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