Effects of lyophilization on the stability of bacteriophages against different serogroups of Shiga toxin-producing Escherichia coli |
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| Affiliation: | 1. Faculty of Pharmacy, University of Sydney, Sydney, NSW 2006, Australia;2. School of Pharmacy, The Chinese University of Hong Kong, Shatin, Hong Kong;3. Department of Mechanical Engineering, University of Alberta, Alberta T6G 1H9, Canada;4. AmpliPhi Biosciences AU, 7/27 Dale Street, Brookvale, Sydney, NSW 2100, Australia;5. Sydney Analytical Vibrational Spectroscopy Facility, and The School of Chemistry, University of Sydney, Sydney, NSW 2006, Australia;6. Tuberculosis Research Program, Centenary Institute and Sydney Medical School, University of Sydney, Sydney, NSW 2006, Australia;7. The Evergreen State College, Olympia, WA 98502, USA;1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China;2. BGI-Qingdao, 2877 Tuanjie Road, Sino-German Ecopark, Qingdao, 266000, China;3. Dalian SEM Bio-Engineering Technology Co. Ltd., Dalian 116620, China;4. Ministry of Education Center for Food Safety of Animal Origin, Dalian 116600, China;1. Biological and Agricultural Engineering, University of California, Davis, One Shields Ave., Davis, CA 95616, USA;2. Food Science and Technology, University of California, Davis, One Shields Ave., Davis, CA 95616, USA;1. Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil;2. Departamento de Ingeniería de Alimentos, Universidade de Córdoba, Colombia;3. Departamento de Física, Universidade Federal de Viçosa, Brazil;4. Departamento de Química, Biotecnologia e Engenharia de Bioprocessos, Universidade Federal de São João Del Rei, Campus Alto Paraopeba − Ouro Branco, Brazil;1. Advanced Drug Delivery Group, School of Pharmacy, University of Sydney, Sydney, NSW, Australia;2. Centenary Institute and Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia;3. AmpliPhi Biosciences AU, Brookvale, Sydney, NSW, Australia;4. The Evergreen State College, Olympia, WA, USA;5. Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, Clayton, Victoria, Australia |
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| Abstract: | Lyophilization is commonly used to effectively preserve the stability of bacteriophages (phages) in long-term storage. However, information regarding the lyophilization of phages specific to Shiga toxin-producing Escherichia coli (STEC) strains is scarce. The objective of this study was to determine the effects of lyophilization with different cryoprotectants (sucrose and trehalose) and concentrations (0.1 M and 0.5 M) on the stability of seven lytic phages specific to STEC O157 and top 6 non-O157 strains during 6-month storage at −80 °C. The titers of lyophilized phages specific to STEC O26 (S1 O26) and STEC O121 (Pr121lvw) did not exhibit significant reduction after 6-month storage regardless of the use of cryoprotectants. Phages lytic against STEC O103 (Ro103C3lw) and STEC O145 (Ro145clw) with 0.1 M sucrose retained similar titers after lyophilization and frozen storage for 6 months (P > 0.05). Despite subtle differences, these results indicated that most of the selected phages had similar titer retention with the same cryoprotectants. Additionally, lytic activities of the phages against their primary hosts were not affected after lyophilization and 6-month frozen storage. Moreover, no detectable damage was observed on the lyophilized phage structures. These findings provide valuable insight into the use of lyophilization to preserve phages lytic against STEC strains. |
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| Keywords: | Bacteriophage Top 6 non-O157 Lyophilization Sucrose Trehalose |
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