Impacts of different synthetic polymers on vitrification of ovarian tissue |
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| Institution: | 1. Laboratory of Diagnostic Imaging Applied to Animal Reproduction, State University of Ceará, Fortaleza, CE, Brazil;2. Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceará, Fortaleza, CE, Brazil;3. Laboratory of Physiology and Control of Reproduction, State University of Ceará, Fortaleza, CE, Brazil;4. Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, Illinois, USA;1. Smithsonian Conservation Biology Institute, National Zoological Park, P.O. Box 37012, MRC 5502, Washington, DC 20008, USA;2. Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853, USA;1. Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;2. Laparoscopic Surgery School of Medicine Endometriosis Research Center Iran University, Tehran, Iran;3. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;4. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;1. Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC 29208, USA;3. Division of Reproductive & Developmental Science, Oregon National Primate Research Center, Beaverton, OR 97006, USA;4. Department of Environmental Science, Center for Reservoir and Aquatic Systems Research, Institute of Biomedical Studies, Baylor University, Waco, TX 76798, USA;5. Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA 30322, USA;6. Department of Obstetrics & Gynecology, Oregon Health & Science University, Portland, OR 97239, USA |
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| Abstract: | Type and concentration of cryoprotective agents (CPAs) are important factors which influence the likelihood of a successful ovarian tissue vitrification outcome. In an attempt to address this factor, the present study was conducted to evaluate the impacts of different synthetic polymers (Supercool X-1000, Supercool Z-1000 and PVP K-12) on vitrification of bovine ovarian tissue. From each ovarian pair, fragments were recovered and immediately fixed for analysis (fresh control) or submitted to vitrification, either or not followed by in vitro culture for one or five days. Vitrification was performed using the ovarian tissue cryosystem (OTC) system. The ovarian tissues were intended for histological and viability analysis Reactive oxygen species (ROS) production and degenerate cells assay (Ethidium homodimer-1)], as well as immunolocalization of AQP3 and AQP9 were measured. The results showed that during almost all the periods after warming, in treatment groups which contain polymer (X-1000, Z-1000 and PVP), the percentage of morphologically normal follicles was the highest in the X-1000 samples. Furthermore, post-thawed X-1000 group revealed stronger labeling for AQP9 in primordial and transitional follicles, when compared with others. However, morphology after cryopreservation did not correlate with follicle viability and function where the levels of degeneration and tissue damage of PVP K-12 group were lower in comparison with X-1000 group and only in PVP K-12 group, ROS level was similar to that of the fresh control group. We believe that in addition to permeating CPAs, the addition of one (Supercool X-1000) or maybe a combination (Supercool X-1000 and PVP K-12) of non-permeating polymers could be useful to improve the outcome for vitrified bovine ovarian tissue. |
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| Keywords: | Ovarian tissue Synthetic polymers Vitrification Cryoprotective agents In vitro culture |
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