Embryo development after vitrification of immature and in vitro-matured equine oocytes |
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| Institution: | 1. Veterinary Sciences Department, Pisa University, San Piero a Grado, Via Livornese, 56124, Pisa, Italy;2. Clinic of Reproductive Medicine, Department for Farm Animals, University of Zurich, Zurich, 8057, Switzerland;1. College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, 77843-4466, United States;2. Laboratory of Animal Reproduction, Faculty of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, MG, 38400-902, Brazil;1. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, QC, Canada;2. Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, 77843, TX, USA;1. Cría Tanoira Embriones, Pilar, Buenos Aires, Argentina;2. Laboratorio de Genética Aplicada, SRA., Cap. Fed., Buenos Aires, Argentina |
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| Abstract: | Effects of meiotic stage and cumulus status on development of equine oocytes after vitrification was evaluated. Immature oocytes with corona radiata (IMM); in vitro-matured oocytes with corona radiata (MAT CR+); and in vitro-matured oocytes denuded of cumulus (MAT CR-) were vitrified using the Cryotech® method. Warming medium was equilibrated either in 5% CO2 or Air. IMM oocytes underwent in vitro maturation after warming. Recovery, survival, and maturation rates, and cleavage and blastocyst rates after ICSI, were evaluated. Recovery was higher for oocytes warmed in CO2- than Air-equilibrated medium (86 ± 3 vs. 76.9 ± 4%, respectively). Maturation for all vitrified-warmed oocyte treatments (37 ± 6.5 to 45.9 ± 5.8%) was not different from control (50 ± 4.1%), except for MAT CR- CO2 (20.3 ± 4.6%). Cleavage for MAT CR- CO2 and Air groups was similar to control (67.7 ± 12.1, 71.4 ± 8.1, and 78 ± 5.3%, respectively). One blastocyst was produced (MAT CR + CO2), representing the first equine blastocyst reported after vitrification of an in vitro-matured oocyte. |
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| Keywords: | Blastocyst Cumulus cells Equine oocyte ICSI Meiotic stage Vitrification |
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