A new material of cryopreserving cell samples |
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| Affiliation: | 1. School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore;2. Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117576, Singapore;1. University of California, Davis Comprehensive Cancer Center, Sacramento, CA;2. The Jackson Laboratory, Bar Harbor, ME and Sacramento, CA;3. Response Genetics, Inc, Los Angeles, CA;1. Genome Center, School of Medicine, University of California at Davis, Davis, CA, USA;2. Population Sciences and Cancer Health Disparities Program, UC Davis Comprehensive Cancer Center, School of Medicine, University of California at Davis, Davis, CA, USA;3. Department of Biochemistry and Molecular Medicine, School of Medicine, University of California at Davis, Davis, CA, USA |
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| Abstract: | Cryopreservation was first studied in early twentieth century, and the cryopreservation of cell and tissue samples has become an inseparable process in biology research labs, helping scientists to store living materials and to accumulate specimens.Recently, a new and simplified cryopreservation product, BioFlash Drive™ SP developed by a US firm Fibulas, came to the attention of many researchers. It integrates the sample container with control-released cryoprotectant, and the container itself can achieve slow-freezing in deep freezers. This means no reagents mixing or extra steps for slow-freezing are needed. The design of this product aims to simplify the current cell-freezing procedures and reduce the lengthy and error prone processes.In this research, we compared the post-thaw cell viability using two of the most widely used protocols, with the one with BioFlash Drive™ SP (Fibulas). Cell lines tested in this research include Vero (ATCC® CCL-81™) and HEp-2 (ATCC® CCL-23™). Results show there is not statistically significant difference in cell viability between the conventional protocols and the new protocols. However, this new protocol is less manual and less time consuming. With this new method, it might be possible for researchers to archive research progress more often, because saving cell can be an easier but still reliable experience. |
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| Keywords: | Cryopreservation Vero HEp-2 Viability Biospecimens Cell freezing |
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