Cryopreservation effects on canine sperm morphometric variables and ultrastructure: Comparison between vitrification and conventional freezing |
| |
| Institution: | 1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, PR China;2. Laboratory of Animal Reproductive Biotechnology, Shanxi Agricultural University, Taigu, 030801, PR China;1. Department of OBGYN, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen Street, Hue, Viet Nam;2. Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen Street, Hue, Viet Nam;3. Department of Histology and Embryology, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen Street, Hue, Viet Nam;1. Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87 –05508-270, São Paulo, Brazil;2. Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, 9000, Belgium;1. Regional Center of Animal Selection and Reproduction (CERSYRA) JCCM, 13300 Valdepeñas, Spain;2. SaBio (IREC) CSIC-UCLM-JCCM, 02071 Albacete, Spain;1. INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France;2. CNRS, UMR7247, F-37380 Nouzilly, France;3. Université François Rabelais de Tours, F-37000 Tours, France;4. IFCE, Institut Français du Cheval et de l’Equitation, F-37380 Nouzilly, France;5. INRA, GenESI, UE 1372 Génétique, expérimentations et systèmes innovants, F-86480 Rouillé, France |
| |
| Abstract: | Semen cryopreservation is an increasingly demanded technique in canids, particularly in order to preserve and spread high genetic value material. Sperm vitrification may represent an interesting alternative to costly and time consuming conventional freezing. The objective of this study was to evaluate the effect of sperm vitrification on sperm morphometry and ultrastructure compared to conventional freezing. Pools of nine beagle dogs were both frozen and vitrified. Computerized morphological parameters (length, wide, area and perimeter) and sperm ultrastructure, using scanning and transmission microscopy, were analysed in both fresh and in thawed/warmed samples. There were no differences (p > 0.05) between post-thaw and fresh morphometric variables of the sperm heads. However, cluster analysis revealed that sperm-heads turned out to be smaller after thawing (p < 0.05) in two of the four subpopulations. Vitrification-warming process led to an overall increase in sperm-head size. Furthermore, the sperm head size increased after warming in two subpopulations (p < 0.05). In conclusion, the variations in the sperm head area depended on the cryopreservation procedure (conventional freezing or vitrification). Conventional freezing tended to decrease the head dimensions, at least in some subpopulations, and vitrification led to an overall increase in the sperm head size. Decondensation of chromatin and plasma membrane blebbing in the head region was observed by transmission electron microscopy in several vitrified sperm, which might explain the increase of head dimensions detected by CASA-Morph system. |
| |
| Keywords: | Spermatozoa Cryopreservation Vitrification Dog |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
