The proteomic characterization of ram sperm during cryopreservation analyzed by the two-dimensional electrophoresis coupled with mass spectrometry |
| |
| Affiliation: | 1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, PR China;2. Laboratory of Animal Reproductive Biotechnology, Shanxi Agricultural University, Taigu, 030801, PR China;1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;2. Animal Husbandry and Veterinary Research Institute of Gansu Province, Pingliang 744000, China;3. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;1. Laboratory of Teaching and Research in Pathology of Reproduction, Center of Biotechnology in Animal Reproduction, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil;2. Laboratory of Andrology and Embryo Technology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil;3. Laboratory of Semen Biotechnology and Andrology, Center of Biotechnology in Animal Reproduction, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, SP, Brazil;4. Laboratory of Andrology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Sao Paulo, SP, Brazil;5. Laboratory of Neuroendocrinology and Reproduction, Department of Physiology, Faculty of Medicine, University of São Paulo, Ribeirao Preto, SP, Brazil |
| |
| Abstract: | The aim of this study was to analyze the effects of the cryopreservation process on the protein profile of ram sperm using two-dimensional electrophoresis (2-DE) coupled with mass spectroscopy. Semen was collected from five rams and cryopreserved in a Tris-based extender supplemented with glycerol and egg yolk as the main cryoprotectants. The fresh and post-thaw sperm total proteins were extracted and purified, followed by the 2-DE. The differential proteins in the stained gel were determined by mass spectrometry. The results indicated that there were 39 differential proteins between fresh sperm and frozen-thawed sperm. Among these proteins, the abundance of 28 proteins in fresh sperm was higher than those in post-thaw sperm (P < 0.05). However, 11 proteins in post-thaw sperm were up-regulated instead. The gene ontology (GO) analysis showed that most of differential proteins were implicated in cellular process, metabolism and regulation of the biological process. The networks of protein-protein interaction indicated a strong interaction among these differential proteins, which may be involved in sperm metabolism, acrosomal function, sperm motility, and reducing ROS level. In conclusion, the cryopreservation process modifies the proteome of ram sperm, which may be directly associated with ram sperm cryodamage, consequently influencing their fertility. Additionally, these differential proteins can be used as biomarkers for evaluation of frozen ram semen quality. |
| |
| Keywords: | Cryodamage Gene ontology analysis Proteome Ram semen Zhaotong rams |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
