The role of mitochondria in shaping odor responses in Drosophila melanogaster olfactory sensory neurons |
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| Institution: | 1. Department of Biological and Chemical Sciences, Illinois Institute of Technology, 3101 S. Dearborn Street, Chicago, IL 60616, USA;2. Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA;3. Department of Neurosciences, Medical University of South Carolina, Charleston, SC 29425, USA;1. Laboratory of Sensory Signaling, Bogomoletz Institute of Physiology, Kyiv, Ukraine;2. Department of Cytology, Bogomoletz Institute of Physiology, Kyiv, Ukraine;3. Laboratory of Molecular Biophysics, Bogomoletz Institute of Physiology, Kyiv, Ukraine;4. International Center for Molecular Physiology, Bogomoletz Institute of Physiology, Kyiv, Ukraine;1. Department of Plant Protection Biology, Division of Chemical Ecology, Swedish University of Agricultural Sciences, Alnarp, Sweden;2. Sørengkaia 115, 0194 Oslo, Norway;3. Zoology Department, Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary;4. Departments of Entomology and Chemistry, University of California, Riverside CA 92521, USA;1. Department of Medical Genetics and Molecular Biochemistry, Lewis Katz School of Medicine at Temple University, Philadelphia, PA 19140, USA;2. Center for Translational Medicine, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, 19140, USA;3. Center for Precision Medicine, Department of Medicine, University of Texas Health San Antonio, San Antonio, Texas 78229;1. Molecular Drug Metabolism and Toxicology Unit, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria;2. Mzima International Research Unit, Kampala, Uganda;3. DrosAfrica, UK |
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| Abstract: | Insects detect volatile chemosignals with olfactory sensory neurons (OSNs) that express olfactory receptors. Among them, the most sensitive receptors are the odorant receptors (ORs), which form cation channels passing also Ca2+. Here, we investigate if and how odor-induced Ca2+ signals in Drosophila melanogaster OSNs are controlled by intracellular Ca2+ stores, especially by mitochondria. Using an open antenna preparation that allows observation and pharmacological manipulation of OSNs we performed Ca2+ imaging to determine the role of Ca2+ influx and efflux pathways in OSN mitochondria. The results indicate that mitochondria participate in shaping the OR responses. The major players of this modulation are the mitochondrial Ca2+ uniporter and the mitochondrial permeability transition pore. Intriguingly, OR-induced Ca2+ signals were only mildly affected by modulating the Ca2+ management of the endoplasmic reticulum. |
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| Keywords: | Insect olfaction Odorant receptor Orco Calcium uniporter Permeability transition pore Calcium imaging |
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