Resveratrol supplementation and cryopreservation of buck semen |
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| Affiliation: | 1. Department of Chemical Engineering, Universidad de La Frontera, Av. Francisco Salazar 01145 Box 54D, Temuco, Chile;2. Center of Biotechnology of Reproduction (CEBIOR), Bioresources Research Nucleus (BIOREN), Universidad de La Frontera, Montevideo 0870, Temuco, Chile;3. School of Aquaculture. Catholic University of Temuco, Av. Rudecindo Ortega 02950, Temuco, Chile;4. Laboratorio de Biotecnología, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, El Líbano 5524, Macul, Santiago, Chile;1. Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran;2. Biomedical Center, Medical Faculty in Pilsen, Charles University in Prague, Alej, Svobody 1655/76, 323 00 Pilsen, Czechia;1. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Physiology, School of Medicine, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran;3. Animal Breeding Center of Iran, Karaj, Iran;4. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;1. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey;2. Department of Biostatistics, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey;3. Department of Pharmacology, Faculty of Medicine, Uludag University, Gorukle/Bursa 16059, Turkey |
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| Abstract: | Resveratrol (Resv) was tested to assess its effects on buck semen freezability. Ejaculates of 4 bucks were collected, washed and diluted in a commercial extender at 30 °C. Extended semen was divided into 4 aliquots supplemented with increasing concentrations of Resv: 0 μM (control); 10 μM; 25 μM and 50 μM. Aliquots were cooled to 4 °C in 5h and frozen in LN2. Thawing was performed at 37 °C for 30 s. At the 3 stages of the experiment (30 °C, 4 °C, thawing), motility (CASA), osmotic resistance (Hos test) and integrity of cytoplasm and acrosome membranes (PI/PSA staining) were assessed. Moreover, in thawed samples, the oxidative status (MDA assay) and early apoptosis (DNA fragmentation by TUNEL assay) were evaluated. Resveratrol supplementation did not affect most of the motility parameters analysed, except for total motility, ALH (lateral head displacement) and velocity distribution (P < 0.05). Functional and morphological integrity of membranes was not affected at any stage of the experiment (P > 0.05). In thawed spermatozoa, the oxidative status was not preserved by Resv (P > 0.05) while early apoptosis, was significantly decreased in the 50 μM Resv group (P < 0.05). Resveratrol did not improve buck semen freezability; the observed effects on motility and DNA were not dose dependent and not mediated by a potential anti-oxidant activity. |
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| Keywords: | Antioxidant Cryopreservation DNA fragmentation Goat Motility Spermatozoa |
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