Resveratrol treatment increases mitochondrial biogenesis and improves viability of porcine germinal-vesicle stage vitrified-warmed oocytes |
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| Affiliation: | 1. Department of Biosciences, Biotechnologies and Biopharmaceutics (DBBB), University of Bari Aldo Moro, Str. Prov. Casamassima Km 3, 70010, Valenzano, Bari, Italy;2. Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Via Manfredonia 20, 71121 Foggia, Italy;3. Obstetrics and Gynecological Section, Department of Veterinary Medicine, Via Vienna, n°2 07100 Sassari, Italy;4. Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), Via G. Amendola 122/O, 70125 Bari, Italy;5. Department of Basical Medical Sciences, Neurosciences and Sensory Organs, University of Bari Aldo Moro, P.zza G. Cesare, 11, 70124 Bari, Italy;6. Institute of Translational Pharmacology (IFT), National Research Council of Italy (CNR) via del Fosso del Cavaliere 100, 00133 Rome, Italy;1. Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China;2. Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea;1. Yunnan Provincial Engineering Laboratory of Animal Genetic Resource Conservation and Germplasm Enhancement, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan 650224, People''s Republic of China;2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan 650201, People''s Republic of China;1. Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon, Korea;2. Institute of Agricultural Science, Chungnam National University, Daejeon, Korea;1. Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China;2. Isotope Research Laboratory, College of Life Science, Sichuan Agricultural University, Ya''an 625014, PR China;3. Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT, USA |
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| Abstract: | Vitrification induces mitochondrial dysfunction in warmed oocytes, and degeneration and biogenesis of mitochondria are crucial for maintaining oocyte quality. The present study addresses a hypothesis that treatment of vitrified-warmed oocytes with resveratrol could improve the viability of oocytes by activating mitochondrial biosynthesis. Cumulus oocyte complexes (COCs) collected from gilt ovaries were vitrified, warmed, and cultured in a medium containing vehicle or 1 μM resveratrol. Resveratrol treatment improved survival, maturation, and mitochondrial membrane potential of vitrified-warmed oocytes, but did not improve the development into blastocysts after parthenogenetic activation. Resveratrol treatment increased mitochondrial synthesis, as determined by the expression levels of TOMM20 and mitochondrial DNA copy number, in vitrified-warmed oocytes, but not in non-vitrified oocytes. In addition, the effect of resveratrol treatment on mitochondrial synthesis was reduced by EX527, a SIRT1 inhibitor. Resveratrol treatment of vitrified-warmed oocytes increased the expression levels of genes involved in mitochondrial synthesis (TFAM, POLG, and PGC1α) and increased nuclear translocation of NRF2. Furthermore, vitrification induced mitophagy, as confirmed by a reduction in TOMM20 expression and decreased p62 aggregation, whereas resveratrol treatment did not affect these mitophagic features. In conclusion, vitrification induced mitochondrial clearance in oocytes, whereas activation of SIRT1 by resveratrol treatment facilitated the recovery of vitrified-warmed oocytes through the activation of mitochondrial synthesis. |
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| Keywords: | Vitrification Mitochondria SIRT1 Resveratrol Porcine oocytes |
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