Addition of butylated hydroxytoluene (BHT) in tris-based extender improves post-thaw quality and motion dynamics of dog spermatozoa |
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| Institution: | 1. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;2. Department of Veterinary Pre-Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, PR China;2. Laboratory of Animal Reproductive Biotechnology, Shanxi Agricultural University, Taigu, 030801, PR China;1. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Faculty of Veterinary Medicine, Department of Clinical Sciences, Shahid Chamran University, Ahvaz, Iran;3. Faculty of Agriculture, Department of Poultry Sciences, Tarbiat Modares University, Tehran, Iran;1. Laboratory of Biotechnology and Pathology of Reproduction, ONIRIS: The National Veterinary, Food Agriculture, and Food Hygiene School of Loire Atlantique, BP 40706, 44307 Nantes, France;2. Laboratory of Biotechnology of Animal Reproduction, SAAD DAHLAB University of Blida (U.BLIDA1), BP 270, 09000 Blida, Algeria;3. UR1268 Biopolymères Interactions Assemblages, Equipe Interfaces et Systèmes Dispersés, INRA, F-44316 Nantes Cedex 3, France;4. IMV Technologies, 10 rue Clemenceau, BP 81, 61302 Aigle Cedex, France;5. Department of Statistics, ONIRIS: The National Veterinary, Food Agriculture, and Food Hygiene School of Loire Atlantique, BP 40706, 44307 Nantes, France |
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| Abstract: | The aim of this study was to evaluate the interaction of different concentrations of butylated hydroxytoluene (BHT) in a tris-based extender on semen quality parameters in post-thawed dog semen. Twenty-four ejaculates were collected from eight male Beagle dogs using an artificial vagina. Pooled semen was diluted with a tris-based extender supplemented with 0 (control), 0.5, 1.0, 1.5, and 2.0 mM BHT, at a final concentration of 200 × 106 spermatozoa/mL. After thawing, sperm samples were assessed for motility parameters (CASA), membrane integrity (SYBR-14/PI), acrosome integrity (FITC-PNA), mitochondrial activity (JC-1/PI), malondialdehyde (MDA) concentration, and glutathione peroxidase (GPx) activity. The total motility, progressive motility, and average path velocity of the frozen-thawed sperm were significantly higher in the BHT1.5 group than in the control and the other sample groups (P < 0.05). Higher values of straight-line velocity, curvilinear velocity, amplitude of the lateral head displacement, and linearity were observed in the BHT1.0, BHT1.5, and BHT2.0 groups than in the control (P < 0.05). The BHT1.0 and BHT1.5 groups had higher percentages of straightness and acrosome integrity than the other groups (P < 0.05). Beat cross frequency, plasma membrane integrity, and GPx activity of the BHT1.5 and BHT2.0 groups were higher than those of the control (P < 0.05). A lower concentration of MDA was observed in the BHT1.0, BHT1.5, and BHT2.0 groups than in the control (BHT0) (P < 0.05).Our results indicate that 1.5 mM BHT is the optimal concentration for improving the post-thaw quality of canine spermatozoa. |
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| Keywords: | Dog sperm Cryopreservation Butylated hydroxytoluene (BHT) Sperm quality Antioxidants |
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