Use of microrespirometry to determine viability of immobilized Burkholderia cepacia G4 |
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Authors: | Santo Domingo Jorge W. Radway JoAnn C. Hazen Terry C. Wilde Edward W. |
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Affiliation: | (1) Westinghouse Savannah River Co., Aiken, South Carolina 29808, USA |
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Abstract: | Embedding of Burkholderia cepacia G4 cells in a polyurethane-based foam decreased their culturability by more than four orders of magnitude. However, respiration rates of immobilized cells were at least 33-41% of unimmobilized cells. Embedded cells also degraded trichloroethylene. Therefore, respirometry is a more reliable indicator of viability of polyurethane immobilized bacteria than culturing methods. |
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