| 上槽泄露导致双向电泳凝胶高分子量区域垂直拖尾 |
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| 引用本文： | 沈立明,兰子尧,任小虎,刘琼. 上槽泄露导致双向电泳凝胶高分子量区域垂直拖尾[J]. 中国生物化学与分子生物学报, 2011, 27(1): 96-100 |
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| 作者姓名： | 沈立明 兰子尧 任小虎 刘琼 |
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| 作者单位： |
（深圳大学生命科学学院，深圳 518060；贵阳医学院公共卫生学院，贵阳 550004） |
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| 基金项目： | 国家自然科学基金项目（No. 20637010, 30570420） |
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| 摘 要： | 本文探讨了电泳系统上槽泄露引起双向电泳凝胶蛋白点纵向拖尾问 题.上槽泄露导致上槽SDS损耗，电流增大以及局部温度升高从而导致双向 电泳凝胶高分子量区域蛋白点纵向拖尾.上槽泄露与上槽设计存在不足，使 用者不适当操作以及上槽维护不良有关.对Ettan DALT six电泳系统而言， 一旦出现上槽泄漏问题，建议更换改良后的新版上槽.电泳过程中应避免上 槽泄露，SDS的不足或损耗，电流过大和电泳液温度过高.
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| 关 键 词： | 双向电泳 垂直拖尾 上槽泄露 |
| 收稿时间： | 2010-07-15 |
Leaking of Buffer from Upper Chamber Caused Vertical Streaking in the High Molecular Weight Separation with 2-DE Gels |
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| SHEN Li-Ming),LAN Zi-Yao),REN Xiao-Hu),LIU Qiong) College of Life Sciences,Shenzhen University,Shenzhen ,China,) School of Public Health,Guiyang Medical University,Guiyang ,China). Leaking of Buffer from Upper Chamber Caused Vertical Streaking in the High Molecular Weight Separation with 2-DE Gels[J]. Chinese Journal of Biochemistry and Molecular Biology, 2011, 27(1): 96-100 |
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| Authors: | SHEN Li-Ming) LAN Zi-Yao) REN Xiao-Hu) LIU Qiong) College of Life Sciences Shenzhen University Shenzhen China ) School of Public Health Guiyang Medical University Guiyang China) |
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| Affiliation: | SHEN Li-Ming1),LAN Zi-Yao2),REN Xiao-Hu1),LIU Qiong1)*(1) College of Life Sciences,Shenzhen University,Shenzhen 518060,China,2) School of Public Health,Guiyang Medical University,Guiyang 550004,China) |
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| Abstract: | This paper intended to solve the problem of vertical streaking caused by the leaking of upper buffer chamber(UBC) in two-dimensional gel electrophoresis(2-DE).UBC leakage resulted in sodium dodecyl sulfate(SDS) depletion,increased current and elevasted local temperature of the upper buffer,leading to vertical streaking in the high-molecular-weight region of 2-DE gels.The leaking problems could be caused by either the defected design of the 2-DE system or the improper usage /maintenance during operation.An i... |
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| Keywords: | two-dimensional gel electrophoresis vertical streaking eaking of the upper buffer chamber |
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