一个可降解直链烷基苯磺酸盐的新种

本文鉴定了一株可完全降解直链烷基苯磺酸盐（Linear Alkylbenzene Sulphonate，简称LAS）的菌株GZ6。革兰氏染色阴性，细胞为杆状或短杆状，大小为0.5μm～0.8 μm×1.0μm～2.0 μm，其生长pH范围为pH6.0～10.0，最适生长pH为7.0，生长温度范围为4℃～40℃，最适生长温度为30℃。生化特征测定除过氧化氢酶、尿酶、精氨酸脱羧酶反应为阳性，其它均为阴性。可利用Chloridazon、安替比林（antipyrin）以及LAS等为碳源。不能利用大多数糖醇。醌组分以泛醌Q10为主。菌体脂肪酸主要为C18∶1、C16∶0及C16∶1。DNA中G+C mol％含量为7010。16S rRNA 序列分析表明菌株GZ6 与其亲缘关系最近的菌株Phenylobacterium immobile DSM1986T序列相似值为9749％，DNADNA杂交率为40%。菌株GZ6具极生鞭毛，可运动，两者在细胞形态有很大差异。故将菌株GZ6定为苯基杆菌属的新种可动苯基杆菌（Phenylobaterium mobile） GZ6。

A New Species of the Genus Phenylobacterium for the Degradation of LAS (Linear Alkylbenzene Sulfonate)

A strain GZ6 that can biodegrade LAS (Linear Alkylbenzene Sulphonate) is identified. It is aerobic gram-negative rod or short-rod (0.5 to 0.8 by 1.0 to 2.0 Mm). It is mobile with a single polar flagellum. Optimum growth occurred at 30 degrees C and pH7.0. It is catalase positive, urease positive, and arginine decarboxylase positive. All the other physiological and biochemical tests performed were negative. It utilizes the xenobiotic compounds chloridazon, antipyrin and LAS as sole carbon sources. Most sugars, alcohols, and carboxylic acids are not utilized. It has Q-10 as the major quinone. The main cell fatty acids are Sum7, C16:0 and Sum4. The DNA G + C mol % content is 70.10. A phylogenic tree was constructed on the basis of 16S rDNA sequences. It showed that the previously known member of the genus Phenylobacterium, Phenylobacterium mobile DSM1986T, is the nearest neighbor to strain GZ6. The level of binary sequence similarity between them is 97.49%. And the DNA-DNA relatedness is 40%. These genetic analysis and their morphological difference show that they are different species of Phenylobacterium. A new species, Phenylobacterium mobile sp. nov., has been proposed.