Network antibodies identify nuclear lamin B as a physiological attachment site for peripherin intermediate filaments |
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Authors: | K Djabali M M Portier F Gros G Blobel S D Georgatos |
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Institution: | College de France, Biochimie Cellulaire, Paris. |
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Abstract: | We studied the molecular associations between peripherin (a neuronal, type III intermediate filament subunit) and nuclear lamins. We show here that isolated peripherin binds selectively to mammalian lamin B under in vitro conditions. We further demonstrate that a synthetic peptide, representing the proximal part of peripherin's tail domain (P1), also associates with mammalian lamin B in a saturable, cooperative, and specific fashion. Laboratory animals immunized with P1 spontaneously develop idiotypic and anti-idiotypic antibodies recognizing peripherin and lamin B, respectively. These data provide essentially in vivo evidence that lamin B represents a constitutive nuclear "receptor" site for the tail domains of peripherin intermediate filaments. |
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