Measurement of nitrite reductase in leaf tissue of Vigna mungo |
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Authors: | R C Srivastava Bandana Bose D Mukerji S N Mathur H S Srivastava |
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Institution: | (1) Department of Botany, University of Gorakhpur, 273001 Gorakhpur, India;(2) Present address: Department of Life Sciences, University of Indore, 452001 Indore, India |
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Abstract: | The enzyme nitrite reductase (EC 1.6.6.4) is generally assayed in terms of disappearance of nitrite from the assay medium. We describe a technique which allowed estimation of the enzyme level in leaf tissues of Vigna mungo (L). Hepper in terms of the release of the product (NH3) of the enzyme reaction. The technique is offered as an alternative, possibly more convenient method for assay of nitrite reductase in plant tissue in vivo. |
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Keywords: | Nitrite reductase Vigna |
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