Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi |
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| Authors: | Horst Vierheilig, Andrew P. Coughlan, Urs Wyss, Yves Pich |
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| Affiliation: | Horst Vierheilig, Andrew P. Coughlan, Urs Wyss, and Yves Piché |
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| Abstract: | We developed a reliable, inexpensive, and simple method for staining arbuscular-mycorrhizal fungal colonizations in root tissues. Apart from applications in research, this nontoxic, high-quality staining method also could be of great utility in teaching exercises. After adequate clearing with KOH, an ink-vinegar solution successfully stained all fungal structures, rendering them clearly visible. |
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