Position specific binding of a monoclonal antibody in chick limb buds |
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| Authors: | K Ohsugi H Ide |
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| Institution: | 1. Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4;2. Department of Molecular Genetics, University of Toronto, Canada M5S 1A8;3. Division of Orthopaedic Surgery, Hospital for Sick Children and University of Toronto, Canada M5G 1X8;1. USAID–Academic Model Providing Access to Healthcare (AMPATH)/Moi Teaching and Referral Moi University School of Medicine, Eldoret 30100, Kenya;2. University of Washington, Seattle, WA, USA;3. National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA;4. Purdue University College of Pharmacy, Indianapolis, IN, USA;5. Webuye District Hospital, Webuye, Kenya;6. Moi University School of Medicine, Eldoret, Kenya |
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| Abstract: | To analyze the molecular mechanism of the limb pattern formation, we have tried to make monoclonal antibodies against antigens from chick limb buds. We obtained one antibody named AV-1 which recognized a specific region of chick limb buds. AV-1 reacted with the distal portion of the anteroventral mesoderm of only developmentally early chick limb buds. Grafts of ZPA region tissue to an anterior site in an embryonic chick wing bud resulted in mirror-image dupliction of the AV-1 antigen region. These data show the possibility that this antigen plays some role in the limb pattern formation. This is the first evidence that a position specific substance really exists in developmentally early limb buds in which the pattern has been considered to be unspecified. |
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