Unexpected improvement in stability and utility of cytochrome c by solution in biocompatible ionic liquids |
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Authors: | Fujita Kyoko Forsyth Maria MacFarlane Douglas R Reid Robert W Elliott Gloria D |
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Affiliation: | School of Chemistry, Monash University, Clayton Victoria 3800, Australia. |
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Abstract: | Proteins generally are only stable in vitro for short periods of time. This results in challenges during isolation and purification of recombinant proteins and reduces the shelf life of protein-based pharmaceuticals. Here we show that certain novel, biocompatible ionic liquids provide a stabilizing solvent for proteins, for example, cytochrome c, such that structure and activity are maintained even after 6 months of storage at room temperature. Normally, this protein would be rendered inactive after only 1 week in buffered aqueous solution. The effect of the ionic liquid solvent appears to be related to protection against hydrolysis. |
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Keywords: | biocompatible ionic liquids protein stability activity secondary structure biopreservation |
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