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R-factor-mediated suppression of the galactose-sensitive phenotype of Escherichia coli K-12 galE mutants |
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| Authors: | Anna Fietta Guido Grandi Massimo Malcovati Gianna Valentini Vittorio Sgaramella Antonio G Siccardi |
| Institution: | 1. Institute of Microbiology, of the University of Pavia Pavia, Italy;2. Institute of Biochemistry, of the University of Pavia Pavia, Italy;3. Institute of Genetics, of the University of Pavia Pavia, Italy;4. CNR Laboratory of Biochemical and Evolutionary Genetics, Pavia, Italy |
| Abstract: | Plasmids S-a and Rts1 suppress the galactose-sensitive phenotype of galE mutants of Escherichia coli K-12, giving rise to both galactose-fermenting and nonfermenting strains. Fermenting strains produce normal inducible UDP-galactose epimerase. Plasmids extracted from either a fermenting or a nonfermenting strain are indistinguishable when examined by either measurements of length of relaxed circular molecules by electron microscopy or electrophoretic pattern of restriction endonuclease digestion products. The phenomenon could be explained by reversible recombination between a plasmid-borne epimerase gene and homologous chromosomal sequences. |
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