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Automated lab-scale production of PVA/PEG-enzyme immobilisates |
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| Authors: | Nora C Bieler Marion B Ansorge-Schumacher Lasse Greiner Dr |
| Institution: | 1. Institute of Chemistry, Enzyme Technology, TU Berlin, Berlin, Germany;2. Institut für Technische und Makromolekulare Chemie, RWTH Aachen University, Aachen, Germany |
| Abstract: | Entrapment of biocatalysts by cryogelation is a gentle method to extend the scope of biocatalysis. To foster the use of this versatile method we devised an automated injector for the production of PVA/PEG beads. The device consists of a thermostated reservoir connected to a programmable injector nozzle and an agitated receiving bath for the droplets. This lab-scale production unit yields up to 1500 beads with immobilized enzyme per minute with a narrow size distribution and good roundness. |
| Keywords: | Automated production Biocatalysis Entrapment Immobilization PVA |