Sucrose phosphorylase as cross-linked enzyme aggregate: Improved thermal stability for industrial applications |
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Authors: | An Cerdobbel Karel De Winter Tom Desmet Wim Soetaert |
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Affiliation: | Centre of Expertise for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium |
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Abstract: | Sucrose phosphorylase is an interesting biocatalyst that can glycosylate a variety of small molecules using sucrose as a cheap but efficient donor substrate. The low thermostability of the enzyme, however, limits its industrial applications, as these are preferably performed at 60°C to avoid microbial contamination. Cross-linked enzyme aggregates (CLEAs) of the sucrose phosphorylase from Bifidobacterium adolescentis were found to have a temperature optimum that is 17°C higher than that of the soluble enzyme. Furthermore, the immobilized enzyme displays an exceptional thermostability, retaining all of its activity after 1 week incubation at 60°C. Recycling of the biocatalyst allows its use in at least ten consecutive reactions, which should dramatically increase the commercial potential of its glycosylating activity. |
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Keywords: | Biocatalysis Cross-linked enzyme aggregate Enzyme immobilization Sucrose phosphorylase Thermostability |
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