Molecular cloning and expression profiles of calreticulin gene from the diamondback moth,Plutella xylostella |
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Authors: | Rye Jin KIM Seung Han OH Mi Young NOH Yong Hun JO Seongon KIM In Seok BANG Yong Seok LEE Iksoo KIM Yeon Soo HAN |
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Institution: | 1. Department of Applied Biology, College of Agriculture and Life Science, Chonnam National University, Gwangju, Korea;2. Jeollanam-do Agricultural Research & Extension Services, Naju, Korea;3. Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University, Asan, Korea;4. Department of Parasitology, College of Medicine and Frontier Inje Research for Science and Technology, Inje, University, Busan, Korea |
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Abstract: | Calreticulin (CRT) plays pivotal roles in Ca2+ homeostasis, molecular chaperoning, infection, inflammation and innate immunity. In an attempt to study the involvement of CRT in innate immunity, the full-length cDNA of calreticulin (PxCRT) was cloned from the diamondback moth, Plutella xylostella. It consists of 1674 bp (excluding poly-A tail) with a longest open reading frame (ORF) of 1197 bp encoding 398 amino acids. In silico analysis of PxCRT ORF reveals that it has various repeat motifs and endoplasmic reticulum retention signal found in all the calreticulin proteins. As expected, high amino acid sequence identities were found from other CRTs identified from Bombyx mori (87%), Galleria mellonella (87%), Apis mellifera (74%), Anopheles gambiae (74%), Tribolium castaneum (73%), Culex quinquefasciatus (73%), Rhodnius prolixus (72%), Nasonia vitripennis (71%), Drosophila melanogaster (71%) and Haemaphysalis qinghaiensis (68%). During development, P. xylostella expressed PxCRT predominantly in the pupal stage. In addition, spatial expression pattern analysis indicates that PxCRT was highly expressed in the silk gland. PxCRT mRNA, furthermore, was strongly induced 3 to 6 h after laminarin treatment, suggesting that PxCRT appears to be involved in immune responses and also plays an important role in the silk gland. |
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Keywords: | calreticulin cDNA expression insect Korea laminarin Plutella xylostella |
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