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Diversity and phylogeny of mitochondrial DNA isolated from mithun Bos frontalis located in Bhutan
Authors:T. Dorji  H. Mannen  T. Namikawa  T. Inamura  Y. Kawamoto
Affiliation:1. Department of Livestock, Ministry of Agriculture, Royal Government of Bhutan, Post Box No. 113, Thimphu, Bhutan.;2. Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.;3. Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.;4. School of Foreign Studies, Aichi Prefectural University, 1522-3 Ibaragabasama, Kumabari, Nagakute-cho, Aichi-gun, Aichi 480-1198, Japan.;5. Primate Research Institute, Kyoto University, 41-2 Kanrin, Inuyama, Aichi 484-8506, Japan
Abstract:We sequenced the 16S rRNA gene in mitochondrial DNA to characterize mithun located in Bhutan and to increase our understanding of its origin. We compared mithun with yak, European cattle, Bhutanese zebu and Indian zebu. Sequencing revealed low nucleotide diversity within the mithun population and their phylogenetic proximity to gaur. A close relationship between Bhutanese mithun and gaur was confirmed by an additional comparison with wild gaur specimens from three locations in Bhutan. Direct domestication of mithun from gaur was supported, while maternal contribution from the cattle lineage during domestication was not supported.
Keywords:Bhutan  gaur  mithun  mitochondrial DNA
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