Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice |
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| Authors: | Florian Krammer Sabine Nakowitsch Paul Messner Dieter Palmberger Boris Ferko Reingard Grabherr Dr |
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| Institution: | 1. Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria;2. Avir Green Hills Biotechnology AG, Vienna, Austria;3. Department of Nanobiotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria |
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| Abstract: | Recent outbreaks of influenza A highlight the importance of rapid and sufficient supply for pandemic and inter-pandemic vaccines. Classical manufacturing methods for influenza vaccines fail to satisfy this demand. Alternatively, cell culture-based production systems and virus-like particle (VLP)-based technologies have been established. We developed swine-origin pandemic H1N1 influenza VLPs consisting of hemagglutinin (A/California/04/2009) and matrix protein. Hemagglutinin and matrix protein were co-expressed in insect cells by the baculovirus expression system. VLPs were harvested from infection supernatants, purified and used for intraperitoneal immunization of BALB/c mice. Immunization induced high serum antibody titers against A/California/04/2009 as well as hemagglutination inhibiting antibodies. Additionally, we compared VLP production in two different insect cell lines, Sf9 and BTI-TN5B1-4 (High Five?). Taken together VLPs represent a potential strategy for the fight against new pandemic influenza viruses. |
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| Keywords: | H1N1 Influenza vaccine Insect cells Pandemic influenza Virus-like particle |
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