Water-in-ionic liquid microemulsion-based organogels as novel matrices for enzyme immobilization |
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Authors: | Ioannis V Pavlidis Kyriakos Tzafestas Haralambos Stamatis Dr |
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Institution: | Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece |
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Abstract: | The use of water-in-ionic liquid microemulsion-based organogels (w/IL MBGs) as novel supports for the immobilization of lipase B from Candida antarctica and lipase from Chromobacterium viscosum was investigated. These novel lipase-containing w/IL MBGs can be effectively used as solid phase biocatalysts in various polar and non-polar organic solvents or ILs, exhibiting up to 4.4-fold higher esterification activity compared to water-in-oil microemulsion-based organogels. The immobilized lipases retain their activity for several hours at 70°C, while their half life time is up to 25-fold higher compared to that observed in w/IL microemulsions. Fourier-transform infrared spectroscopy data indicate that immobilized lipases adopt a more rigid structure, referring to the structure in aqueous solution, which is in correlation with their enhanced catalytic behavior observed. |
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Keywords: | Biocatalysis Immobilization Ionic liquids Lipase Microemulsions |
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