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Microspectrofluorometry of the autofluorescent flagellum in phototactic brown algal zoids
Authors:Hiroshi Kawai  Shago Nakamura  Mamoru Mimuro  Masaki Furuya  Masakatsu Watanabe
Affiliation:(1) Kobe University Research Center for Inland Seas, Rokkodai, 657 Kobe, Japan;(2) Department of Environmental Biology and Chemistry, Faculty of Science, Toyama University, Toyama;(3) National Institute for Basic Biology, Okazaki;(4) Advanced Research Laboratory, Hitachi Ltd., Saitama
Abstract:Summary Posterior flagella of zoids ofScytosiphon lomemaria andChorda filum (Phaeophyceae, Chromophyta) were isolated and subjected to microspectrofluorometry using a high sensitivity microspectrofluorometer in order to characterize the flagellar autofluorescent substances. Vigorous agitation in a Hypertonic medium yielded preparations of largely intact flagella retaining detectable green flagellar autofluorescence. Under 380–425 nm excitation light, maximum emission of flagellar autofluorescence was observed at 495 nm, whereas under 400–440 nm excitation light fluorescence shifted to around 510 nm. Comparison of these spectra with the fluorescence spectra of 4prime,5prime-cyclic FMN isolated from fertile thalli ofS. lomentaria, and of 6-carboxypterin suggested that two (or more) different fluorescent substances (presumably a flavin and a pterin) are present in the flagella.Abbreviations DTT dithiothreitol - FMN flavin mononucleotide - HEPES N-[2-hydroxyethyl]piperazine-Nprime-[2-ethanesulfonic acid]) - PEG polyethylene glycol - PFB paraflagellar body - Tris tris(hydroxymethyl) aminomethane.Dedicated to Professors Masakazu Tatewaki and Tadao Yoshida on the occasion of their academic retirement
Keywords:Chorda filum  Flagellar fluorescence  Flavin  Microspectrofluorometry  Phototaxis  Scytosiphon lomentaria
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