In vitro shoot proliferation and propagation of guava (Psidium guajava L.) from germinated seedlings |
| |
Authors: | Yasseen Mohamed-Yasseen Sheryl A Barringer Raymond J Schnell Walter E Splittstoesser |
| |
Institution: | 1. Department of Horticulture, S-406 Turner Hall, University of Illinois, 1102 S. Goodwin Ave., 61801, Urbana, IL, USA 2. Department of Food Sci. & Tech., 122 Vivian Hall, Ohio State University, 2121 Fyffe Rd., 43210-1097, Columbus, OH, USA 3. USDA, ARS, Nat. Colonal Germplasm Rep., 13601 Old Cutler Rd., 33158, Miami, FL, USA
|
| |
Abstract: | Guava seeds were germinated on Murashige and Skoog (MS) medium with or without 8.8 μM benzyladenine (BA). BA increased the rate of germination and the number of lateral shoots (3.4 vs 1.2 per seedling). Stem nodes from these lateral shoots were cultured on proliferation media with 4.4 μM BA, and multiple shoots (3.5) were formed within 4 weeks of culture. Increasing the concentration of BA or the addition of naphthaleneacetic acid (NAA) did not affect shoot formation. Shoots produced from explants and lateral shoots from germinated seedlings were rooted in media containing activated charcoal (AC) or 9.8 μM indolebutyric acid (IBA). Shoots rooted with IBA had a higher rooting percentage (100% vs 75%) and a greater number of roots (5.5 vs 3.2) but the shoots were shorter (2.6 vs 3.4 cm) than when rooted in AC, and they required an additional 4 weeks of culture in media with AC to achieve shoot elongation. About 80% of the shoots with roots survived in the glasshouse and produced normal phenotypic plants. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|