Ferric ion-induced lipid peroxidation in erythrocyte membranes: effects of phytic acid and butylated hydroxytoluene |
| |
| Authors: | Kam Ming Ko David V. Godin |
| |
| Affiliation: | (1) Department of Pharmacology & Therapeutics, Faculty of Medicine, The University of British Columbia, 2176 Health Sciences Mall, V6T 1W5 Vancouver, B. C., Canada |
| |
| Abstract: | Ferric ion was found to stimulate the peroxidation of erythrocyte membrane lipids, causing a biphasic and concentration-dependent increase in the formation of thiobarbituric acid reactive substances. Ascorbic acid and reduced glutathione were able to enhance this lipid peroxidation, presumably by facilitating the reduction of ferric ion. Iron chelators, such as phytic acid, ethylenediaminetetraacetic acid and uric acid, and the chain-reaction-terminating antioxidant butylated hydroxytoluene suppressed the ferric ion-induced peroxidation by actions not likely related to hydroxyl radical scavenging. The effectiveness of phytic acid, a naturally occurring antioxidant, in the inhibition of iron-dependent lipid peroxidation suggests its possible therapeutic application as a non-toxic iron chelator for ameliorating the extent of oxy-radical-induced tissue damage.Abbreviations BHT Butylated Hydroxytoluene - EDTA Ethylenediaminetetraacetic Acid - GSH Reduced Glutathione - TBA 2-Thiobarbituric Acid - TBARS 2-Thiobarbituric Acid Reactive Substances |
| |
| Keywords: | ferric ion erythrocyte membranes lipid peroxidation phytic acid butylated hydroxytoluene |
| 本文献已被 SpringerLink 等数据库收录！ |
|
