DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication |
| |
Authors: | Ariumi Yasuo Kuroki Misao Abe Ken-ichi Dansako Hiromichi Ikeda Masanori Wakita Takaji Kato Nobuyuki |
| |
Affiliation: | Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Okayama 700-8558, Japan. |
| |
Abstract: | DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|