| 浙江省境内乌药种群遗传多样性研究 |
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| 引用本文： | 顾婧婧,金则新,李钧敏.浙江省境内乌药种群遗传多样性研究[J].植物研究,2010,30(2):202-207. |
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| 作者姓名： | 顾婧婧 金则新 李钧敏 |
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| 作者单位： | 1.西南大学三峡库区生态环境教育部重点实验室，重庆 400715；;2.台州学院生态研究所，临海 317000 |
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| 基金项目： | 浙江省自然科学基金资助(Y507660) |
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| 摘 要： | 利用ISSR分子标记方法对浙江省境内的8个乌药自然种群的遗传多样性和遗传分化进行了研究。从100个引物中筛选出12个用于正式扩增的ISSR引物,在8个种群160个个体中共检测到125个位点,其中多态位点100个,多态位点百分率(P)为80%,各种群P平均为60.5%。乌药总的Shannon信息指数(I)为0.3762,Nei指数(h)为0.246 4,各种群I平均为0.317 3,h平均为0.213 1。AMOVA分析表明在总的遗传变异中,11.44%的变异发生在种群间,88.56%的变异发生于种群内,种群间的遗传分化系数(Gst)为0.134 9,乌药种群间的基因流(Nm)为3.205 2。8个种群间的平均遗传距离为0.049 4,利用算术加权平均数法(UPGMA)对乌药8个种群进行聚类,结果可分为2大类群,台州市的水磨坑、上泮、苍山等3个种群聚为一类,其余5个种群聚为另一类。
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| 关 键 词： | 乌药 遗传多样性 遗传分化 ISSR |
Genetic Diversity of Lindera aggregata Populations in Zhejiang Province |
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| GU Jing-Jing,JIN Ze-Xin,LI Jun-Min.Genetic Diversity of Lindera aggregata Populations in Zhejiang Province[J].Bulletin of Botanical Research,2010,30(2):202-207. |
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| Authors: | GU Jing-Jing JIN Ze-Xin LI Jun-Min |
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| Institution: | 1.Key laboratory of Eco-environments in Three Gorges Reservoir Region’s(Ministry of Education),Southwest China University,Chongqing 400715；2.Institute of Ecology,Taizhou University,Linhai 317000 |
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| Abstract: | Eight populations of Lindera aggregata were collected from Zhejiang Province and the genetic diversity and genetic differentiation were analyzed by using inter-simple sequence repeat(ISSR) molecular makers.12 relaible primers were selected from total 100 primers and 125 loci were detected in 160 individuals.100 loci were polymorphic and the percentage of polymorphic loci(P) at species level was 80% with an average of 60.5% at population level.The genetic diversity estimated by Shannon information index(I) a... |
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| Keywords: | Lindera aggregate genetic diversity genetic differentiation ISSR |
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