斜纹夜蛾核多角体病毒gp37及其邻近序列的克隆及分析

从斜纹夜蛾核多角体病毒(Spodoptera litura nucleopolyhedrovirus, SpltMNPV)基因组中克隆了gp37基因.分子生物学软件分析表明，在gp37基因内部存在糖基化位点.含有晚期表达基因的启动子序列(ATAAG)，推测为晚期表达的病毒膜糖蛋白基因.通过GP37蛋白的同源性比较，绘制了以gp37基因为基础的杆状病毒进化树, 发现与以多角体蛋白基因（polyhedrin）为基础所绘制的杆状病毒分子进化树有较大差异.以polyhedrin基因为基础绘制的杆状病毒分子进化树将家蚕核多角体病毒（Bombyx mori nucleopolyhedrovirus, BmNPV）与杆状病毒代表种——苜蓿丫纹夜蛾核多角体病毒（Autographa californica multinucleocapsid nucleopolyhedrovirus, AcMNPV）分开，根据gp37基因分析则将二者归到同一分枝，这与以egt基因为基础绘制的杆状病毒分子进化树结果一致.

Cloning and Sequencing of Spodoptera litura Multicapsid Nucleopolyhedrovirus gp37 Gene Cluster

The gp37 genes of Spodoptera litura NPV (SpltMNPV) was cloned and sequenced. Employing computer analysis, glycosilated sites and the promoter motif existed in the gp37 gene. and was shown as a late gene encoding a 37 ku glycoprotein. A phylogeny tree was designed by the comparison of their homologues from known gp37 genes of baculoviruses. It was shown that the phylogeny of baculoviruses on the basis of gp37 gene was different from that based on polyhedrin genes. For example, Bombyx mori NPV (BmNPV) and Autographa californica NPV(AcMNPV) were evolved in the same branch, otherwise, they were put in two distinct branches in the tree according to the analysis of polyhedrin. This result was consistent with the baculovirus phylogeny based on EGT published previously.