Celery transformation by Agrobacterium tumefaciens: cytological and genetic analysis of transgenic plants |
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| Authors: | D. Catlin O. Ochoa S. McCormick C. F. Quiros |
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| Affiliation: | (1) Department of Vegetable Crops, University of California, 95616 Davis, CA, USA;(2) Plant Gene Expression Center, USDA-ARS, 94710 Albany, CA, USA |
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| Abstract: | Transgenic celery plants were obtained following co-cultivation of petiole explants with Agrobacterlum tumefaciens containing pMON200, a cointegrate vector carrying genes for kanamycin resistance and nopaline synthase. Transformants were selected by ability of callus to grow in the presence of 50mg/l kanamycin. Transformation was confirmed either by the presence of nopaline or by Southern blots. Cytological analysis of 14 transformed plants revealed chromosomal aberrations, both in structure and number. Only 20% of the regenerated plants had the normal karyotype. Kanamycin resistance behaved as a monogenic, dominant trait, segregating in a 3:1 ratio in three families derived from plants with normal karyotypes.Abbreviations KB Kilobases - 2-4D 2,4-diphenoxyacetic acid |
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