Effect of 12C+5 ion beam irradiation on cell viability and plant regeneration in callus,protoplasts and cell suspensions of Lavatera thuringiaca |
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| Authors: | Alejandro Vazquez-Tello Takeshi Uozumi Makoto Hidaka Yasuhiko Kobayashi Hiroshi Watanabe |
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| Affiliation: | (1) Department of Biotechnology, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113 Tokyo, Japan;(2) Japan Atomic Energy Research Institute, Takasaki Radiation Chemistry Research Establishment, Watanuki-machi 1233, 370-12 Takasaki, Japan;(3) Present address: Département des Sciences Biologiques, Universitè du Québec à Montréal, C.P. 8888, Succ. Centre-Ville, H3C 3P8 Montreal, Canada |
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| Abstract: | Summary The biological effects of irradiation with12C+5 ion beam on plant cells have been analyzed. Protoplasts and cell suspensions ofLavatera thuringiaca, and a somatic hybrid callus (Hibiscus rosa-sinensis +Lavatera thuringiaca), were irradiated with doses from 0.05 to 50 Gy, and the effects on cell growth, cell division, cell viability and embryogenesis rates were analyzed. Irradiation with12C+5 ion beam at relatively very low doses (5.0 Gy) significantly inhibited cell division, yet the survival rate and regeneration capability of the cells through somatic embryogenesis were conserved in more than 70 and 50 %, respectively. These results indicate that cell division is the most sensitive parameter to irradiation, accounting for the inhibition of colony formation and callus growth. The potential use of the12C+5 ion beam in asymmetric protoplast fusion experiments is discussed. |
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| Keywords: | Ion beam irradiation Somatic embryogenesis Somatic hybridization Hibiscus rosa-sinensis Lavatera thuringiaca |
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