首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Control of the pyridine nucleotide-linked Ca release from mitochondria by respiratory substrates
Authors:Vladimir Gogvadze  Matthias Schweizer  Christoph Richter  
Institution:Laboratory of Biochemistry I, Swiss Federal Institute of Technology (ETH), Zürich, Switzerland
Abstract:Oxidation of mitochondrial pyridine nucleotides followed by their hydrolysis promotes Ca2+ release from intact liver mitochondria. In most of the previous studies oxidation was achieved with pro-oxidants which were added to mitochondria respiring on succinate in the presence of rotenone, a site I-specific inhibitor of the respiratory chain. Here we investigate pro-oxidant dependent and independent Ca2+ release from mitochondria when respiration is supported either by the NAD+-linked substrate β-hydroxybutyrate, or by succinate. In the presence, as well as in the absence, of the pro-oxidant t-butylhydroperoxide mitochondria retain Ca2+ much better with succinate than with β-hydroxybutyrate, as respiratory substrate. When Ca2+ release is induced by t-butylhydroperoxide succinate-supported Ca2+ retention is impeded by rotenone. Ca2+ release (pro-oxidant dependent or independent) is paralleled by oxidation and hydrolysis of intramitochondrial pyridine nucleotides, and Ca2+ retention is paralleled by reduction of pyridine nucleotides. It is concluded that the pyridine nucleotide-linked Ca2+ release from mitochondria can be controlled by respiratory substrates which regulate the intramitochondrial hydrolysis of oxidized pyridine nucleotides.
Keywords:Abbreviations: CSA  cyclosporine A  EGTA  ethylene glycol bis((β-aminoethylether)-N  N  N′  N′-tetraacetic acid  HEPES  4-(2hydroxyethyl)-1-piperazine ethanesulfonic acid  tbh  t-butylhydrope roxide  Tris  tris(hydroxymethyl)aminomethane
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号