Strict paternal transmission of mitochondrial DNA of Chlamydomonas species is explained by selection against maternal nucleoids |
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Authors: | Nakamura S Aoyama H van Woesik R |
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Institution: | (1) Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, JP;(2) Department of Biological Sciences, Florida Institute of Technology, Melbourne, Florida, US |
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Abstract: | Summary. The non-Mendelian inheritance of organelle DNA is common in most plants and animals. Here we examined inheritance mechanisms
involved in the transfer of mitochondrial DNA. We successively backcrossed (to F5) two interfertile strains of the unicellular isogamous haploid algae Chlamydomonas reinhardtii and Chlamydomonas smithii to match nuclear backgrounds and examine transmission patterns of mitochondrial DNA by PCR analysis of cob gene sequences. Mitochondrial DNA was strictly transmitted paternally. To investigate the behavior of parental mitochondrial
DNA, we used F5 progeny to form zygotes and isolated single zygotes. The results showed selective disappearance of maternal mitochondrial
nucleoids occurred between 3 and 6 h after zygote formation.
Received July 11, 2002; accepted September 28, 2002; published online June 13, 2003
RID="*"
ID="*" Correspondence and reprints: Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus,
Nishihara, Okinawa 903-0213, Japan. |
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Keywords: | : Non-Mendelian inheritance Paternal transmission Chlamydomonas spp Backcross Mitochondrial DNA Nested PCR |
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