Adenosine Transport into Guinea-pig Synaptosomes |
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Authors: | C Barberis A Minn J Gayet |
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Institution: | Laboratoire de Physiologic Générale, Universitéde Nancy 1, Nancy 54037, France |
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Abstract: | Abstract: Kinetics for transport of adenosine into guinea-pig neocortex synaptosomes were studied by incubating them with 14C]adenosine for up to 30 s. The apparent K m value of the high-affinity transport system for adenosine was 21.1 μM and the V max value was 257.3 pmol/min/mg protein. The transport system was inhibited by both compounds structurally related (compounds 554 and 555) and unrelated (dipyridamole) to adenosine. Because electrically stimulated synaptosomes release up to 1.5% of the adenosine derivative content per min, the physiological significance of adenosine uptake is discussed as a possible mechanism to compensate for the loss of adenine nucleotides from synaptosomes preparations. |
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Keywords: | Adenosine Transport Synaptosomes Guinea pig |
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