高分子聚合物HFMC注入猕猴输精管后附睾的远期组织病理学变化

本实验采用７只成年雄性猕猴，经双侧阴囊上方切口暴露输精管，由近附睾端向远侧注射高分子聚合物ＨＦＭＣ，剂量分别为３０ｍｇ／侧（１只），６０ｍｇ／侧（３只），１００ｍｇ／侧（３只），分别于术后２．５及３．５年处死动物，取附睾组织进行光镜及电镜观察。结果表明：注射不同剂量ＨＦＭＣ２．５年后，动物附睾头、体、属各种光镜观察所见主要改变为上皮细胞局灶性轻度水样变性，少许上皮细胞脱落，个别管腔扩张，局部间质少

LONG-TERM HISTO-PATHOLOGICAL CHANGES OF RHESUS MONKEY EPIDIDYMIDES BY INTRA-VASA DEFERENS INJECTION OF HFMC

Seven male rhesus monkeys were used for the investigation of the histopathological changes of epididymides in the 2. 5th and 3.5th years after intra-vasa deferens injection of high molecular polymer HFMC. Various dosages of HFMC (Hydrogel for male contraception) were given to the animals: 30mg per vas deferens for 1 animal, 60mg for 3 and 100mg for 3.The light microscopic observation showed that there were slight local watery degeneration of epididymal epithelium, falling of some epithelial cells and expansion of lumen in some epididymal tubules in the 2.5th year following intra vas injection of HFMC but no obvious abnomality was found under light microscope in the 3.5th year.The main ultrastructure changes in the 2.5th year showed the swelling of mitochondria, slight vacuolar change of endoplasmic reticulum and damage of sperms in the epididymal tubles. The ultrastructure changes in the 3.5th year were similar to those in the 2.5th year.It is suggested that HFMC may directly or indirectly affect the epididymal structure and the epididymal enviroment due to its gradual release of H+ as well as its partial and temporary occlusion of vasa deferens. This may be one of the reasons for its male contraceptive action. However, it seems that pathological may be restored after HFMC is progressively dissolved and excereted.