The occurrence and taxonomic value of PBS X-like defective phages in the genus Bacillus |
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| Authors: | H Y Steensma L A Robertson J D van Elsas |
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| Affiliation: | (1) Laboratory of Microbiology, Delft University of Technology, Julianalaan 67a, Delft, The Netherlands;(2) Present address: Instituto de Microbiología da UFRJ, Centro de Ciencias da Saude, Ilha da Fundão-Bloco 1, 20 000 Rio de Janeiro, RJ, Brasil |
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| Abstract: | 72 strains of 24 Bacillus species were induced with mitomycin C. The lysates were examined for the presence of defective phages resembling PBS X in morphology. All strains tested of B. amyloliquefaciens. B, licheniformis, B. pumilus and B. subtilis contained such phages. Five morphological types of defective, PBS X-like phage could be distinguished, differing in their tail lengths and in the number of cross-striations on the tail. The quaternary structure of the tail, the molecular weight of the main tail protein and the antigenic properties of the phages were identical. The killing ranges of the defective phages have been determined and their possible use in taxonomy discussed. |
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