Purification and neuroprotective effects of polysaccharides from Opuntia Milpa Alta in cultured cortical neurons |
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Authors: | Yang ChenBo Zhao Xiaolan HuangJinjin Zhan Yan ZhaoMei Zhou Lianjun Guo |
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Affiliation: | a Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei 430030, PR China b Chinese National Analytical Centre, Guangdong Institute of Analysis, Guangzhou 510070, PR China |
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Abstract: | Opuntia is a traditional plant from China with medicinal applications. In this experiment, polysaccharides from Opuntia Milpa Alta (MAPs) were analyzed using gas chromatograph-mass spectrometer (GC-MS) method and result showed that MAPs contained mannose (6.37%), rhamnose (14.94%), xylose (1.99%), arabinose (24.07%), galactose (38.25%), ribose (2.63%) and glucose (11.48%). The neuroprotective effects of MAPs were evaluated at the mechanistic level in vitro models of cerebral ischemic injury. In vitro oxygen/glucose deprivation (OGD) model, MAPs (0.5 μg/ml, 5 μg/ml, 50 μg/ml) effectively increased cell viability by methyl thiazolyl tetrazolium (MTT) assay, inhibited cell cytotoxicity by lactate dehydrogenase (LDH) assay, reduced neuronal cell death, suppressed the production of intracellular reactive oxygen species (ROS), decreased of intracellular free Ca2+ concentrations ([Ca2+]i), and reduced extracellular glutamate level. Therefore, MAPs might prevent intracellular calcium overload and decreased glutamate excitotoxicity, both of which can cause neuronal injury and death in vitro models of cerebral ischemic injury. |
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Keywords: | Opuntia Milpa Alta Polysaccharides Neuroprotection ROS Calcium Glutamate |
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