4-(Ethoxycarbonyl) phenyl-1-amino-oxobutanoic acid-chitosan complex as a new matrix for silver nanocomposite film: preparation, characterization and antibacterial activity

The present work describes the preparation of new chitosan complex with 4-(ethoxycarbonyl) phenyl-1-amino-oxobutanoic acid (ETHA), as a matrix for silver nanoparticles to obtain a nanocomposite film by solution casing method. The characterization of the prepared nanocomposite film was made by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetry (TG) and scanning electron microscopy (SEM). The optical property of nanocomposite film was analyzed by UV-Visible and photo-luminescence (PL) spectroscopy. The nanocomposite film was screen for antibacterial activity with Staphylococcus aureus (gram positive), Pseudomonas aurigionasa (gram negative) and Escherichia coli (gram negative) bacteria by adopting the disk diffusion method. The result of antibacterial study revealed that the prepared nanocomposite film may be a promising candidate for wide range of bio-medical applications.