A general solution for the steady-state kinetics of immobilized enzyme systems |
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Authors: | D. C. Rees |
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Affiliation: | (1) Department of Chemistry and Biochemistry, Molecular Biology Institute, University of California, 90024 Los Angeles, CA, USA |
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Abstract: | A power series solution is presented which describes the steady-state concentration profiles for substrate and product molecules in immobilized enzyme systems. Diffusional effects and product inhibition are incorporated into this model. The kinetic consequences of diffusion limitation and product inhibition for immobilized enzymes are discussed and are compared to kinetic behavior characteristic of other types of effects, such as substrate inhibition and substrate activation. |
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