1-(5-Isoquinolinesulfonyl)-2-methylpiperazine (H-7) is a selective inhibitor of protein kinase C in rabbit platelets |
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Authors: | S Kawamoto H Hidaka |
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Institution: | Department of Pharmacology, Mie University School of Medicine, Tsu 514, Japan |
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Abstract: | Effects of 1-(5-isoquinolinesulfonyl)-2-methylpeperazine (H-7), a potent inhibitor of protein kinase C in vitro (1), were investigated with regard to stimulus-induced protein phosphorylation of rabbit platelets. While H-7 inhibited the protein kinase C-mediated phosphorylation in 12-0-tetradecanoylphorbol-13-acetate (TPA)-stimulated platelets, this compound did not block the Ca2+-calmodulin-dependent phosphorylation in Ca2+ ionophore A23187-stimulated cells. This selective inhibitor of protein kinase C, in intact cells, will facilitate studies on the biological functions of protein kinase C. |
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Keywords: | EGTA ethylene glycol bis (β-aminoethyl ether)-N N N′ N′-tetraacetic acid SDS sodium dodecyl sulfate TPA 12-0-tetradecanoylphorbol-13-acetate H-7 1-(5-isoquinolinesulfonyl)-2-methylpiperazine |
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