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Isolation of an amylose-free starch mutant of the potato (Solanum tuberosum L.) |
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| Authors: | J. H. M. Hovenkamp-Hermelink E. Jacobsen A. S. Ponstein R. G. F. Visser G. H. Vos-Scheperkeuter E. W. Bijmolt J. N. de Vries B. Witholt W. J. Feenstra |
| Affiliation: | (1) Department of Genetics, University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands;(2) Department of Biochemistry, University of Groningen, Nijenborgh 16, NL-9747 AG Groningen, The Netherlands |
| Abstract: | Summary An amylose-free potato mutant was isolated after screening 12,000 minitubers. These minitubers had been induced on stem segments of adventitious shoots, which had been regenerated on leaf explants of a monoploid potato clone after Röntgen-irradiation. The mutant character is also expressed in subterranean tubers and in microspores. Starch granules from the mutant showed a strongly reduced activity of the granule bound starch synthase and loss of the major 60 kd protein from the starch granules. |
| Keywords: | Solanum tuberosum Mutant Starch composition Granule-bound starch synthase amylose-free |
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