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| 桂西南早熟荔枝实生资源遗传多样性的ISSR分析 | |
| 引用本文： | 沈庆庆,朱建华,彭宏祥,何新华.桂西南早熟荔枝实生资源遗传多样性的ISSR分析[J].广西植物,2013,33(2):225-228. |
| 作者姓名： | 沈庆庆 朱建华 彭宏祥 何新华 |
| 作者单位： | 1. 广西大学,农学院,南宁,530004;玉林市农业科学研究所,广西,玉林,537000;广西作物遗传改良生物技术重点开放实验室,南宁,530007 2. 广西农业科学院,园艺研究所,南宁,530007 3. 广西大学,农学院,南宁,530004 |
| 基金项目： | 国家荔枝龙眼产业技术体系育种岗位"熟期育种"(项目编号：CARS-33-03)广西农业科学院基本科研业务专项(项目编号：桂农科2013YT03) |
| 摘 要： | 利用ISSR标记技术对83份早熟荔枝(品种)单株遗传多样性进行分析。筛选出多态性高的10条IS-SR引物,共扩增出128条DNA条带,其中多态性带107条,多态性百分率为83.59%,表明桂西南早熟荔枝实生资源遗传多样性较丰富;用NTSYS软件计算出这83份材料的DICE相似系数在0.64～0.95之间,遗传亲缘关系较近;用UPGMA方法构建分子树状图,在相似系数0.75时,可将栽培品种与桂西南早熟实生单株区分开。各地区资源混杂聚类在一起,不能按地区单独聚类。 |
| 关 键 词： | 早熟荔枝 实生资源 遗传多样性 ISSR |
Genetic diversity of early-maturing seedling litchi resources in southwest Guangxi by ISSR |
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| SHEN Qing-Qing,ZHU Jian-Hu,PENG Hong-Xiang,HE Xin-Hua.Genetic diversity of early-maturing seedling litchi resources in southwest Guangxi by ISSR[J].Guihaia,2013,33(2):225-228. | |
| Authors: | SHEN Qing-Qing ZHU Jian-Hu PENG Hong-Xiang HE Xin-Hua |
| Institution: | 1.College of Agriculture, Guangxi University, Nanning 530004, China; 2.Institute of Horticulture, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3.Yulin Institute of Agricultural Sicence, Yulin 537000, China; 4.Guangxi Key lab of Crop Genetic Improvement and Biotechnology, Nanning 530007, China |
| Abstract: | The genetic diversities of 83 early-maturing litchi(variety) plants were examined by using ISSR markers in this study. Ten primers gave reproducible,polymorphic DNA amplification patterns with a total of 128 amplified bands. The percentage of polymorphic band was 83.59%,which showed that the early-maturing seedling litchi resources in southwest Guangxi had abundant genetic diversity. The genetic similarity ranged from 0.64 to 0.95 based on DICE. A dendrogram was constructed by UPGMA method,and the early-maturing litchi(variety)plants were distinguished from cultivar litchi at a similarity coefficient criterion of 0.75. No significant correlation between the dendrogram and geographical distance was revealed among the resources. |
| Keywords: | early-maturing litchi seedling litchi resources genetic diversity ISSR markers |
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