Effects of thiol protease inhibitors on intracellular degradation of exogenous beta-galactosidase in cultured human skin fibroblasts |
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Authors: | Y M Ko T Yamanaka M Umeda Y Suzuki |
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Institution: | 1. Department of Pediatrics, Faculty of Medicine, University of Tokyo, Tokyo, Japan;2. Division of Chemical Pathology, National Center for Nervous, Mental and Muscular Disorders, Tokyo, Japan;3. Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan |
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Abstract: | The effects of low molecular weight (LMW) protease inhibitors of microbial origin were evaluated on the intracellular degradation of beta-galactosidase purified from Aspergillus oryzae and taken up by cultured human skin fibroblasts with beta-galactosidase deficiency. Only thiol protease inhibitors showed an effect to increase the enzyme activity. E-64, a specific inhibitor of thiol proteases, prolonged 3-fold a half life of the exogenous beta-galactosidase and when the enzyme was supplied as liposomes, the half life was prolonged 9-fold in these cells. The role of thiol proteases in the degradation of enzyme molecules was discussed. |
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