Caloric restriction influences hydrogen peroxide generation in mitochondrial sub-populations from mouse liver |
| |
Authors: | Kevork Hagopian Yana Chen Keira Simmons Domer Robert Soo Hoo Trevor Bentley Roger B McDonald Jon J Ramsey |
| |
Institution: | (1) Department of Animal Physiology-II, Complutense University, c/Antonio Novais-2, Madrid, 28040, Spain;(2) Department of Basic Medical Sciences, University of Lleida, Lleida, 25008, Spain; |
| |
Abstract: | Calorie restriction (CR) has been shown to decrease H2O2 production in liver mitochondria, although it is not known if this is due to uniform changes in all mitochondria or changes
in particular mitochondrial sub-populations. To address this issue, liver mitochondria from control and CR mice were fractionated
using differential centrifugation at 1,000 g, 3,000 g and 10,000 g into distinct populations labeled as M1, M3 and M10, respectively.
Mitochondrial protein levels, respiration and H2O2 production were measured in each fraction. CR resulted in a decrease in total protein (mg) in each fraction, although this
difference disappeared when adjusted for liver weight (mg protein/g liver weight). No differences in respiration (State 3
or 4) were observed between control and CR mice in any of the mitochondrial fractions. CR decreased H2O2 production in all fractions when mitochondria respired on succinate (Succ), succ+antimycin A (Succ+AA) or pyruvate/malate+rotenone
(P/M+ROT). Thus, CR decreased reactive oxygen species (ROS) production under conditions which stimulate mitochondrial complex
I ROS production under both forward (P/M+ROT) and backward (Succ & Succ+AA) electron flow. The results indicate that CR decreases
H2O2 production in all liver mitochondrial fractions due to a decrease in capacity for ROS production by complex I of the electron
transport chain. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|