The Androctonus australis garzoni scorpion venom contains toxins that selectively affect voltage-dependent K+-channels in cerebellum granular cells |
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| Authors: | Marzia Pisciotta Fredy I Coronas Lourival D Possani G Prestipino |
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| Institution: | (1) Istituto di Cibernetica e Biofisica, Consiglio Nazionale delle Ricerche, Via de Marini 6, I-16149 Genova, Italy (e-mail: presti@barolo.icb.ge.cnr.it), IT;(2) Instituto de Biotechnologia/UNAM, Avenida Universidad 2001, Cuernavaca 62271, Mexico, MX |
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| Abstract: | A purified peptide from Androctonus australis Garzoni venom (AaG) affects selectively a K+-current recorded from cerebellum granular cells. This current is characterized by fast activating and inactivating kinetics
similar to an IA-type current. Addition of 2 μm peptide Aa1 (from Androctonus australis, toxin 1) to the external side of the channel suppressed completely and in a selective manner the IA-type current, with an IC50 value of 130 nm, whereas in the same conditions, the other potassium current, identified as delayed rectifier (Id), was not affected. Additionally, we show that another partially purified peptide (III-12) from the same venom was able to
block reversibly both K+-currents.
Received: 10 February 1997 / Accepted: 7 August 1997 |
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| Keywords: | Scorpion venom Granular cells Patch-clamp Potassium currents |
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