| STAT3 入核分子机制的初步研究 |
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| 引用本文： | 叶中德,沈倍奋,宋伦.STAT3 入核分子机制的初步研究[J].生物工程学报,2004,20(2):299-301. |
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| 作者姓名： | 叶中德 沈倍奋 宋伦 |
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| 作者单位： | 军事医学科学院基础医学研究所，北京100850 |
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| 基金项目： | 国家杰出青年科学基金(No.3995019);国家973项目(No.001CB510005)资助. |
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| 摘 要： | 为了研究Stat3入核的分子机制，将SV40大T抗原的经典核定位序列NLS(nuclear localization sequence)分别融合在Stat3-GFP分子和缺失突变体Dstat3-GFP的分子之间，构建Stat3-NLS-GFP和Dstat3-NLS-GFP融合分子。转染293T细胞，以NLS-GFP为阳性对照，通过激光共聚焦显微镜的观察融合分子的亚细胞位置，未经白介素-6刺激的Stat3-NLS-GFP和经白介素-6刺激的Stat3-GFP呈胞核分布，未经白介素-6刺激的Stat3-GFP和Dstat3-NLS-GFP呈胞浆分布，初步证明Stat3入核是由于获得了核定位序列。
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| 关 键 词： | Stat3 细胞核 入核分子机制 核定位序列 NLS 白介素-6 Stat3-NLS-GFP Dstat3-NLS-GFP 融合分子 |
| 文章编号： | 1000-3061(2004)02-0299-03 |
| 修稿时间： | 2003年8月14日 |
The Mechanism of Stat3 Nuclear Import |
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| YE Zhong\|De , SHEN Bei-Fen SONG Lun.The Mechanism of Stat3 Nuclear Import[J].Chinese Journal of Biotechnology,2004,20(2):299-301. |
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| Authors: | YE Zhong\|De SHEN Bei-Fen SONG Lun |
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| Institution: | Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. |
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| Abstract: | In order to investigate the mechanism of stat3 nuclear import. positio ned a characterized NLS of the SV40 large T antigen into Stat3\|GFP ,Dstat3\|GF P respectively between the C\|terminus of Stat3 and the N\|terminus of GFP to cr eate Stat3\|NLS\|GFP and Dstat3\|NLS\|GFP. With NLS\|GFP as the positive contro l, Expression of the Stat3\|NLS\|GFP without IL\|6 stimulation and Stat3\|GFP wi th IL\|6 stimulation resulted in a predominantly nuclear localization in 293T ce ll. Expression of Stat3\|GFP and Dstat3\|NLS\|GFP without IL\|6 stimulation resu lted in predominantly cytoplasm localization in 293T cell. The results suggest that latent Stat3 is not anchored in the cytoplasm, and that nuclear localizatio n in response to IL\|6 is facilitated by gain of an NLS function. |
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| Keywords: | Stat3 nuclear import NLS |
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