Substitutions at auxiliary operator O3 enhance repression by nitrate-responsive regulator NarL at synthetic lac control regions in Escherichia coli K-12 |
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| Authors: | Stewart Valley Bledsoe Peggy J |
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| Institution: | Section of Microbiology, University of California, One Shields Avenue, Davis CA 95616-8665, USA. vjstewart@ucdavis.edu |
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| Abstract: | We constructed monocopy lac operon control regions in which the operators O1-lac and O3-lac were replaced by NarL and NarP binding sites from the nirB or napF operon control regions. The results support the hypothesis that DNA-bound dimers of phospho-NarL can participate in higher-order cooperative interactions. |
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